Regulatory
Part:BBa_K2587006:Design
Designed by: Ylenia Longo Group: iGEM18_Duesseldorf (2018-09-15)
Plas
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4
Illegal BsaI.rc site found at 140
Design Notes
The sequence contains Type II S restriction sites and is optimised for the use with the CIDAR toolbox.
Source
This sequence is derived from Genbank and is codon optimised for E. coli.